A fast isocratic liquid chromatography method for the quantification of xanthophylls and their stereoisomers

作者：Nimalaratne Chamila; Lopes Lutz Daise; Schieber Andreas; Wu Jianping^*

来源：Journal of Separation Science, 2015, 38(24): 4166-4172.

DOI：10.1002/jssc.201500656

• Geometric isomers
• Isocratic elution
• Xanthophylls

A fast isocratic liquid chromatography method was developed for the simultaneous quantification of eight xanthophylls (13-Z-lutein, 13'-Z-lutein, 13-Z-zeaxanthin, all-E-lutein, all-E-zeaxanthin, all-E-canthaxanthin, all-E-beta-apo-8'-carotenoic acid ethyl ester and all-E-beta-apo-8'-carotenal) within 12 min, compared to 90 min by the conventional high-performance liquid chromatography method. The separation was achieved on a YMC C-30 reversed-phase column (100 mm x 2.0 mm; 3 mu m) operated at 20 degrees C using a methanol/tert-butyl methyl ether/water solvent system at a flow rate of 0.8 mL/min. The method was successfully applied to quantify lutein and zeaxanthin stereoisomers in egg yolk, raw and cooked spinach, and a dietary supplement. The method can be used for the rapid analysis of xanthophyll isomers in different food products and for quality control purposes.

• 出版日期2015-12