A fungicidal piperazine-1-carboxamidine induces mitochondrial fission-dependent apoptosis in yeast

作者：Bink Anna; Govaert Gilmer; Francois Isabelle E J A; Pellens Klaartje; Meerpoel Lieven; Borgers Marcel; Van Minnebruggen Geert; Vroome Valerie; Cammue Bruno P A^*; Thevissen Karin

来源：FEMS Yeast Research, 2010, 10(7): 812-818.

DOI：10.1111/j.1567-1364.2010.00663.x

• apoptosis
• fungicidal
• lipid raft
• caspase
• mitochondrial fission machinery
• piperazine-1-carboxamidine

To unravel the working mechanism of the fungicidal piperazine-1-carboxamidine derivative BAR0329, we found that its intracellular accumulation in Saccharomyces cerevisiae is dependent on functional lipid rafts. Moreover, BAR0329 induced caspase-dependent apoptosis in yeast, in which the mitochondrial fission machinery consisting of Fis1 (Whi2), Dnm1 and Mdv1 is involved. Our data are consistent with a prosurvival function of Fis1 (Whi2) and a proapoptotic function of Dnm1 and Mdv1 during BAR0329-induced yeast cell death.

• 出版日期2010-11