A Conserved Mechanism for Centromeric Nucleosome Recognition by Centromere Protein CENP-C

作者：Kato Hidenori; Jiang Jiansheng; Zhou Bing Rui; Rozendaal Marieke; Feng Hanqiao; Ghirlando Rodolfo; Xiao T Sam; Straight Aaron F; Bai Yawen^*

来源：Science, 2013, 340(6136): 1110-1113.

DOI：10.1126/science.1235532

Chromosome segregation during mitosis requires assembly of the kinetochore complex at the centromere. Kinetochore assembly depends on specific recognition of the histone variant CENP-A in the centromeric nucteosome by centromere protein C (CENP-C). We have defined the determinants of this recognition mechanism and discovered that CENP-C binds a hydrophobic region in the CENP-A tail and docks onto the acidic patch of histone H2A and H2B. We further found that the more broadly conserved CENP-C motif uses the same mechanism for CENP-A nucteosome recognition. Our findings reveal a conserved mechanism for protein recruitment to centromeres and a histone recognition mode whereby a disordered peptide binds the histone tail through hydrophobic interactions facilitated by nucleosome docking.

• 出版日期2013-5-31