Adenosine signaling pathway regulated bladder fibrosis caused by outlet obstruction in rats

作者：Long, Zhi; Shi, Xuan-Yan; Tang, Jin; Dai, Ying-Bo; Wen, Jia-Ming; Tang, Yu-Xin; Zhang, Yi-Chuan; He, Le-Ye^*

来源：International Journal of Clinical and Experimental Pathology, 2016, 9(3): 3277-3285.

• Bladder fibrosis
• bladder outlet obstruction
• adenosine
• adenosine receptor

This study is to investigate the role of adenosine signaling pathway in bladder fibrosis caused by outlet obstruction. A bladder fibrosis model was established in female Wistar rats. Immunohistochemistry and real-time fluorescent quantitative polymerase chain reaction (RT-PCR) were used to measure the changes of fibrosis-related gene expression in bladder tissues, including collagen-I, collagen-III, transforming growth factor-beta 1 (TGF-beta 1), and ecto-5'-nucleotidase (CD73) genes. Adenosine receptor blocker theophylline was injected intraperitoneally to confirm the potential role of adenosine in the pathogenesis of bladder fibrosis. A surgery sham and a normal control groups were also included in the study. Compared with the normal control and sham groups, the expression levels of collagen-I, collagen-III, and TGF-beta 1 genes in the bladder outlet obstruction (BOO) group were significantly increased, while CD73 gene expression was significantly decreased (P < 0.05). After injection of theophylline, the expressions of collagen-I, collagen-III, and TGF-beta 1 genes were significantly decreased, while CD73 gene was significantly upregulated (P < 0.05). In conclusions; Adenosine signaling pathway plays an important role in the pathogenesis of bladder fibrosis caused by BOO. These findings suggest a potential intervention for bladder fibrosis in patients with a similar condition, such as benign prostatic hyperplasia.

• 出版日期2016
• 单位浙江大学; 中南大学