Analytical evaluation of Sysrnex UF-1000i for flow cytometric analysis of peritoneal fluid

作者：Buoro Sabrina; Gustinetti Rosanna; Dominoni Paola; Alessio Maria Grazia; Filisetti Patrizia; Luca Maria Grazia; Fagiuoli Stefano; Ottomano Cosimo; Lippi Giuseppe^*

来源：Clinical Biochemistry, 2012, 45(15): 1263-1265.

DOI：10.1016/j.clinbiochem.2012.05.005

• Peritoneal fluid
• Flow cytometly
• Analytical performance
• Evaluation

Objective: To evaluate analytical performance of Sysmex UF-1000i for peritoneal fluid analysis. %26lt;br%26gt;Methods: Functional sensitivity, imprecision, linearity and comparison studies were performed on peritoneal fluids. %26lt;br%26gt;Results: Total imprecision was 1.6-4.7%, functional sensitivity 27/mu L for white blood cell (WBC) and 3244 for total nucleated cell (TNC) count. Linearity was excellent up to 983 cell/mu L, carry-over %26lt; 0.2%, correlation with manual microscopy always greater than 0.992. %26lt;br%26gt;Conclusion: The instrument exhibited optimal performance at the conventional WBC diagnostic thresholds.

• 出版日期2012-10