Adeno-associated virus Rep78 restricts adenovirus E1B55K-mediated p53 nuclear exportation

作者：Wang Jingjing; Li Wenjuan; Wang Ran; Xue Jinglun; Chen Jinzhong^*

来源：Acta Biochimica et Biophysica Sinica, 2013, 45(2): 135-140.

DOI：10.1093/abbs/gms097

• p53
• Rep78
• E1B55K
• interaction

Inactivation of p53 is needed during adenovirus type 5 DNA replication. E1B55K, an adenovirus early protein, has been reported to interact with p53 and inhibit p53 transactivation. Previous studies have shown that adeno-associated virus (AAV) type 2 could reduce the transforming potential of adenovirus by rescuing p53 from adenovirus-mediated degradation, but the details are not clear yet. We detected the Rep78p53 interaction by co-immunoprecipitation assay. The co-localization assay revealed that Rep78 inhibits E1B55K-mediated p53 nuclear exportation. However, Rep78 did not detectably influence p53 stability and could not relieve the transcriptional inactivation of p53, as E1B55K could not be replaced from the p53E1B55K complex by Rep78. Our results reveal a new possible mechanism that AAV-2 Rep78 inhibits adenovirus 5 by relocalizing p53 in the nucleus, which may shed some light on the regulatory mechanism of AAV-2 on its helper virus, adenovirus.

• 出版日期2013-2
• 单位复旦大学