Sixty years from discovery to solution: crystal structure of bovine liver catalase form III

作者：Foroughi Leila M; Kang You Na; Matzger Adam J^*

来源：Acta Crystallographica Section D-Biological Crystallography, 2011, 67: 756-762.

DOI：10.1107/S0907444911024486

The crystallization and structural characterization of bovine liver catalase (BLC) has been intensively studied for decades. Forms I and II of BLC have previously been fully characterized using single-crystal X-ray diffraction. Form III has previously been analyzed by electron microscopy, but owing to the thinness of this crystal form an X-ray crystal structure had not been determined. Here, the crystal structure of form III of BLC is presented in space group P2(1)2(1)2(1), with unit-cell parameters a = 68.7, b = 173.7, c = 186.3 angstrom. The asymmetric unit is composed of the biological tetramer, which is packed in a tetrahedron motif with three other BLC tetramers. This higher resolution structure has allowed an assessment of the previously published electron-microscopy studies.

• 出版日期2011-9