科研之友
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摘要
In vitro antioxidant activity of stem extracts of Justicia gendarussa Burm along with in vivo hepatoprotective activity of methanolic fraction was carried out to ascertain the folkloric claim of its hepatoprotective activity. The crude methanolic extract was prepared by soxhlet extraction and fractionated into pet ether, chloroform and methanol fractions. The marc remained was further extracted with doubled distilled water by refluxation in waterbath. Preliminary phytochemical tests, total phenolic and flavonoid content present in each fraction/extract was determined. All fractions and aqueous extract were evaluated for their antioxidant activity using DPPH free radical scavenging activity, hydrogen peroxide scavenging activity, reduction of ferric ion in presence and absence of EDTA. The methanolic fraction was further studied for its in vivo hepatoprotective activity using CCl(4) induced hepatotoxicity in albino rats. The various biochemical parameters were evaluated to asses its hepatoprotective activity. Methanolic fraction has more phenolic/flavonoid content and shows good antioxidant activity. The methanolic fraction has a good hepatoprotective activity at dose of 300 mg kg(-1) b.wt. in albino rats. Interestingly its hepatoprotective activity decreases as the dose increases. Stem extract of JG has moderate hepatoprotective activity; it may be due to its total phenolic and flavonoid contents. These findings substantiate the ancient literature about its reports as hepatoprotective herb and inspires for further extensive study regarding its exact hepatoprotective activity.
- 出版日期2010-4
