Anti-inflammatory and anti-angiogenic activities of a purified polysaccharide from flesh of Cipangopaludina chinensis

作者：Xiong, Qingping; Hao, Hairong; He, Lian; Jing, Yi; Xu, Tingting; Chen, Jing; Zhang, Haijiang; Hu, Tao; Zhang, Qianghua; Yang, Xue^*; Yuan, Jun^*; Huang, Yu

来源：Carbohydrate Polymers, 2017, 176: 152-159.

DOI：10.1016/j.carbpol.2017.08.073

• Cipangopaludina chinensis
• Edible flesh
• Polysaccharide
• Anti-inflammatory activity
• Anti-angiogenic activity

The purpose of this paper was to investigate the anti-inflammatory and anti-angiogenic activities of a purified polysaccharide (CCPSn) from flesh of Cipangopaludina chinensis in lipopolysaccharide (LPS) activated RAW264.7 macrophages and human umbilical vein endothelial cells (HUVECs), respectively. Anti-inflammatory results showed that CCPS not only greatly decreased the pro -/anti-inflammatory cytokine secretion ratios, including TNF-alpha/IL-10, IL-6/IL-10 and IL-1 beta/IL-10, but also reduced release levels of nitric oxide (NO) and prostaglandin E-2 (PGE(2)), and suppressed expressions of cyclooxygenase2 (COX2) and inducible nitric oxide synthase (iNOS). The results from anti-angiogenic activities revealed that proliferation, migration, tube formation and vascular endothelial growth factor (VEGF) of HUVECs were significantly inhibited by CCPSn treatment. Even more remarkable was the fact that anti-inflammatory and anti-angiogenic activities of CCPSn exhibited a clear dose response manner, and these activities were irrelevant to the cytotoxicities of CCPSn to RAW264.7 macrophages and HUVECs. These results indicated that CCPSn possessed strong anti-inflammatory and anti-angiogenic activities.

• 出版日期2017-11-15
• 单位淮阴工学院; 徐州医科大学; 广东食品药品职业学院