An accurate and reliable real time SNP genotyping assay for the HLA-G +3142 bp C %26gt; G polymorphism

作者：Bortolotti D; Gentili V; Melchiorri L; Rotola A; Rizzo R^*

来源：Tissue Antigens, 2012, 80(3): 259-262.

DOI：10.1111/j.1399-0039.2012.01926.x

• 3%26apos
• untranslated region
• human leukocyte antigen-G
• miRNA
• single-nucleotide polymorphism genotyping

Human leukocyte antigen (HLA)-G is a non classical HLA class I antigen with immuno-modulatory functions. The HLA-G gene is characterized by a +3142C%26gt;G variant in the 3%26apos; untranslated region which is suggested to control protein production and to be associated with pathological conditions. DNAs form 221 randomly selected healthy subjects were genotyped for HLA-G +3142C%26gt;G polymorphism by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) (BaeGI), real-time PCR and sequencing. The 19% of the PCR-RFLP heterozygous samples were genotyped as 3142GG by real-time PCR and sequencing. This disagreement is caused by digestion efficiency in PCR-RFLP. This real-time PCR method will guarantee an accurate genotyping for future research and clinical purposes, where large cohorts should be tested.

• 出版日期2012-9