ESAM is a novel human hematopoietic stem cell marker associated with a subset of human leukemias

作者：Ishibashi Tomohiko; Yokota Takafumi^*; Tanaka Hirokazu; Ichii Michiko; Sudo Takao; Satoh Yusuke; Doi Yukiko; Ueda Tomoaki; Tanimura Akira; Hamanaka Yuri; Ezoe Sachiko; Shibayama Hirohiko; Oritani Kenji; Kanakura Yuzuru

来源：Experimental Hematology, 2016, 44(4): 269-281.

DOI：10.1016/j.exphem.2015.12.010

Reliable markers are essential to increase our understanding of the biological features of human hematopoietic stem cells and to facilitate the application of hematopoietic stem cells in the field of transplantation and regenerative medicine. We previously identified endothelial cell-selective adhesion molecule (ESAM) as a novel functional marker of hematopoietic stem cells in mice. Here, we found that ESAM can also be used to purify human hematopoietic stem cells from all the currently available sources (adult bone marrow, mobilized peripheral blood, and cord blood). Multipotent colony-forming units and long-term hematopoietic-reconstituting cells in immunodeficient mice were found exclusively in the ESAM(High) fraction of CD34(+)CD38(-) cells. The CD34(+)CD38(-) fraction of cord blood and collagenase-treated bone marrow contained cells exhibiting extremely high expression of ESAM; these cells are likely to be related to the endothelial lineage. Leukemia cell lines of erythroid and megakaryocyte origin, but not those of myeloid or lymphoid descent, were ESAM positive. However, high ESAM expression was observed in some primary acute myeloid leukemia cells. Furthermore, KG-1a myeloid leukemia cells switched from ESAM negative to ESAM positive with repeated leukemia reconstitution in vivo. Thus, ESAM is a useful marker for studying both human hematopoietic stem cells and leukemia cells.

• 出版日期2016-4