Molecular characterisation of Klebsiella oxytoca strains isolated from patients with antibiotic-associated diarrhoea

作者：Alikhani Mohammad Yousef; Shahcheraghi Fereshteh; Khodaparast Sepideh; Nejad Amir Sasan Mozaffari; Moghadam Mojgan K; Mousavi Sayed Fazlollah^*

来源：Arab Journal of Gastroenterology, 2016, 17(2): 95-101.

DOI：10.1016/j.ajg.2016.03.005

• Klebsiella oxytoca
• Antibiotic-associated colitis
• Cytotoxin

Background and study aim: Colitis is a common complication after treatment with antibiotics such as quinolones, and aminoglycosides. Recently, Klebsiella oxytoca has been implicated in this type of diarrhoea. The prevalence and characterisations of K. oxytoca isolated from patients with antibiotic associated diarrhoea were investigated. The K. oxytoca isolates were also tested for cytotoxin production.
Patients and methods: This study was conducted from May 2011 to Dec 2013. Faecal samples were collected from hospitalised patients receiving antibiotic treatment. Initial cultivation was performed on specific media. The clinical isolates were confirmed by polymerase chain reaction (PCR) using the specific K. oxytoca polygalacturonase (pehX) gene. The double-disc diffusion test was used to detect extended spectrum beta-lactamase (ESBL)-producing strains. Tracking of ESBL-encoding genes was performed via PCR. The organism was cultured on Hep-2 cell lines for cytotoxin production.
Results: Out of 331 samples collected from patients, 40 were confirmed molecularly to be clinical isolates of K oxytoca. Fourteen (35%) ESBL-producing strains were isolated using the double -disc diffusion method. Among the molecularly confirmed K oxytoca isolates, seven (17.5%) tested positive for the bIaSHV gene, 12 (30%) for bIaTEM, 10 (25%) for blaCTX-M, three (7.5%) for blaOXA, nine (22.5%) for b/aCTX-M-15, and seven (17.5%) for blaTEM-1. Five (12%) isolates showed cytotoxin activity below 30%, 12 (30%) strains showed moderate cytotoxin activity between 30% and 60%, and 23 (58%) strains showed cytotoxin activity 2:60%.
Conclusions: The cytotoxin-producing K oxytoca is found to be one of the causes of antibiotic-induced colitis. Discontinuing treatment and allowing normal intestinal flora to be established or prescribing appropriate medication after antibiogram can help patients with antibiotic-induced haemorrhagic colitis in a timely manner.

• 出版日期2016-6