Nonlinear spectral imaging of human normal skin, basal cell carcinoma and squamous cell carcinoma based on two-photon excited fluorescence and second-harmonic generation

作者：Xiong, S. Y.^*; Yang, J. G.; Zhuang, J.

来源：Laser Physics, 2011, 21(10): 1844-1849.

DOI：10.1134/S1054660X11170269

• MULTIPHOTON MICROSCOPY
• ENDOGENOUS FLUORESCENCE
• OPTICAL MICROSCOPY
• SPECTROSCOPY
• COMBINATION
• DERMIS

In this work, we use nonlinear spectral imaging based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) for analyzing the morphology of collagen and elastin and their biochemical variations in basal cell carcinoma (BCC), squamous cell carcinoma (SCC) and normal skin tissue. It was found in this work that there existed apparent differences among BCC, SCC and normal skin in terms of their thickness of the keratin and epithelial layers, their size of elastic fibers, as well as their distribution and spectral characteristics of collagen. These differences can potentially be used to distinguish BCC and SCC from normal skin, and to discriminate between BCC and SCC, as well as to evaluate treatment responses.

• 出版日期2011-10
• 单位福建医科大学