A Molecular Network for the Transport of the TI-VAMP/VAMP7 Vesicles from Cell Center to Periphery

作者：Burgo Andrea^*; Proux Gillardeaux Veronique; Sotirakis Emmanuel; Bun Philippe; Casano Alessandra; Verraes Agathe; Liem Ronald K H; Formstecher Etienne; Coppey Moisan Maite; Galli Thierry

来源：Developmental Cell, 2012, 23(1): 166-180.

DOI：10.1016/j.devcel.2012.04.019

The compartmental organization of eukaryotic cells is maintained dynamically by vesicular trafficking. SNARE proteins play a crucial role in intracellular membrane fusion and need to be targeted to their proper donor or acceptor membrane. The molecular mechanisms that allow for the secretory vesicles carrying the v-SNARE TI-VAMP/VAMP7 to leave the cell center, load onto microtubules, and reach the periphery to mediate exocytosis are largely unknown. Here, we show that the TI-VAMP/VAMP7 partner Varp, a Rab21 guanine nucleotide exchange factor, interacts with GolginA4 and the kinesin 1 Kif5A. Activated Rab21-GTP in turn binds to MACF1, an actin and microtubule regulator, which is itself a partner of GolginA4. These components are required for directed movement of TI-VAMP/VAMP7 vesicles from the cell center to the cell periphery. The molecular mechanisms uncovered here suggest an integrated view of the transport of vesicles carrying a specific v-SNARE toward the cell surface.

• 出版日期2012-7-17