Phytoglycoprotein (75 kDa) Suppresses Release of Histamine and Expression of IL-4 and IFN- gamma in BPA-treated RBL-2H3 Cells

作者：Park Cheol Ho; Lim Kye Taek^*

来源：Immunological Investigations, 2010, 39(2): 171-185.

DOI：10.3109/08820130903513449

• Phytoglycoprotein (75 kDa)
• PKC alpha
• NF-kappa B
• Histamine
• Interleukin-4
• Interferon-gamma

The current study investigated whether the phytoglycoprotein (75 kDa) isolated from Cudrania tricuspidata Bureau (CTB glycoprotein) has anti-allergic inflammatory potential. Bisphenol A (BPA) is well known to promote development of allergy-related immune dysfunction. This experiment evaluated the release of histamine, increased intracellular Ca<SU2+</SU level, and the activities of protein kinase C alpha (PKC alpha), transcription factors NF kappa B and cytokines (IL-4, and IFN-gamma) in BPA-treated RBL-2H3 cells. The results in this study showed that the phytoglycoprotein (75 kDa) inhibits the intracellular Ca<SU2+</SU level, translocation of PKC alpha from cytosol to membrane and the translocation of NF-kappa B in cells. We also found that the phytoglycoprotein (75 kDa) inhibits the release of histamine, and the expressions of cytokines (IL-4 and IFN-gamma) in the presence of BPA in RBL-2H3 cells. The results obtained from these experiments indicated that the phytoglycoprotein (75 kDa) inhibits release of histamine and expressions of IL-4, and IFN-gamma via down regulations of Ca<SU2+</SU/PKC alpha and NF-kappa B on the stage of degranulation induced by BPA. From point of view, we speculated that the phytoglycoprotein (75 kDa) is one of natural compounds that can block allergic-inflammation caused by BPA.</.

• 出版日期2010