Adjuvant PIKA protects hepatoma cells from dengue virus infection by promoting a TBK-1-dependent innate immune response

作者：Zhang, Ping; Wu, Siyu; Li, Lietao; Liang, Zhaoduan; Li, Yuye; Feng, Lianqiang; Huang, Xi^*

来源：Archives of Virology, 2013, 158(4): 829-838.

DOI：10.1007/s00705-012-1556-8

• TIME RT-PCR
• INTERFERONS
• INDUCTION
• MICE
• VACCINE
• POTENT
• MODEL
• TLR3

Our study presents a first investigation of the effect of the adjuvant PIKA on dengue virus (DENV) replication. PIKA pretreatment decreased the levels of DENV serotype 2 (DENV2) mRNA, protein and viral particles in the hepatoma cell line HepG2. Treatment with PIKA simultaneously with DENV2 infection, but not after infection, resulted in a protective effect. Significant induction of type I and type III interferons (IFNs), as well as interferon-stimulated genes was detected in PIKA-pretreated cells. Neutralization of IFN-beta partially restored the replication levels of DENV2 in PIKA-pretreated cells, suggesting that IFN-beta is one of the mediators involved in the antiviral action of PIKA. Additionally, blockade of TBK-1 signaling largely restored the IFN induction and viral suppression effects mediated by PIKA, further illustrating that PIKA plays its anti-DENV role by promoting innate immunity. These findings suggest that PIKA is an attractive agent to be used in the prevention of DENV diseases.

• 出版日期2013-4
• 单位中山大学