Association of estrogen with glucocorticoid levels in visceral fat in postmenopausal women

作者：Yamatani Hizuru; Takahashi Kazuhiro^*; Yoshida Takayuki; Takata Keiko; Kurachi Hirohisa

来源：Menopause-The Journal of the North American Menopause Society, 2013, 20(4): 437-442.

DOI：10.1097/gme.0b013e318271a640

• Estrogen
• 17 beta-Hydroxysteroid dehydrogenase
• 11 beta-Hydroxysteroid dehydrogenase
• Menopause
• Cortisol

Objective: The aim of this study is to clarify the association between estrogen and glucocorticoid levels in adipose tissues of premenopausal and postmenopausal women. %26lt;br%26gt;Methods: Forty (15 premenopausal and 25 postmenopausal) women aged 22 to 79 years were recruited through elective gynecological surgical operation, and both subcutaneous and visceral fat were collected during the surgical operation. Messenger RNA (mRNA) expressions of 11 beta hydroxysteroid dehydrogenase type 1 (HSD1), which catalyzes cortisone to cortisol, and 17 beta-HSD1 and 17 beta hydroxysteroid dehydrogenase type 2 (HSD2), which catalyze the interconversion of estrone (E-1) and estradiol (E-2), were measured by real-time reverse transcription polymerase chain reaction. The levels of E-1, E-2, cortisol, and cortisone in adipose tissues were measured by liquid chromatography tandem mass spectrometry. %26lt;br%26gt;Results: The visceral fat area was significantly increased (P %26lt; 0.01) in postmenopausal women compared with that in premenopausal women. The cortisol/cortisone ratio (P %26lt; 0.01) and the expression of 11 beta-HSD1 mRNA (P %26lt; 0.001) in visceral fat, but not in subcutaneous fat, in postmenopausal women were significantly higher than those in premenopausal women. There was a significant correlation (r(2) = 0.69, P %26lt; 0.05) between the E-1/E-2 ratio of visceral fat and body mass index in postmenopausal women. A significant correlation (r(2) = 0.54, P %26lt; 0.05) between the cortisol/cortisone ratio and the E-1/E-2 ratio of visceral fat was observed in postmenopausal women. %26lt;br%26gt;Conclusions: The expression of 11 beta-HSD1 mRNA in visceral fat increases in postmenopausal women, and the E-1/E-2 ratio in visceral fat may be associated with local glucocorticoid levels after menopause.

• 出版日期2013-4