Delayed gastric emptying and altered antrum protein metabolism during activity-based anorexia

作者：Nobis S; Morin A; Achamrah N; Belmonte L; Legrand R; Chan P; do Rego J L; Vaudry D; Gourcerol G; Dechelotte P; Goichon A; Coeffier M^*

来源：Neuro-Gastroenterology and Motility, 2018, 30(7): e13305.

DOI：10.1111/nmo.13305

• activity-based anorexia
• gastric emptying
• malnutrition
• protein metabolism

BackgroundAnorexia nervosa, a restrictive eating disorder, is often associated with gastrointestinal disorders, particularly a delayed gastric emptying. However, the mechanisms remained poorly documented. Thus, we aimed to evaluate gastric emptying and antrum protein metabolism in the Activity-Based Anorexia model (ABA).
MethodsFemales C57Bl/6 mice were randomized into 3 groups: Control, ABA, and Limited Food Access (LFA). Food access has been progressively limited from 6h/day at day 6 to 3h/day at day 9 and until day 17. ABA mice had free access to an activity wheel. Gastric emptying was assessed. On gastric extracts, a proteomic analysis was performed, as well as an evaluation of protein synthesis and protein oxidation.
Key ResultsBoth LFA and ABA mice exhibited a delayed gastric emptying compared with Controls (P<.05). Proteomic approach revealed 15 proteins that were differentially expressed. Among these proteins, we identified 2 clusters of interest contributing to (i) the organization of muscle fiber with ACTA2, VCL, KRT19, KRT8, and DES proteins and (ii) heat shock proteins with STIP1, HSPD1, and HSPA8 proteins. ABA mice specifically exhibited an increased rate of gastric oxidized proteins.
Conclusions and InferencesDelayed gastric emptying observed in anorectic conditions appears to be secondary to malnutrition. However, an oxidative stress is specifically present in the stomach of ABA mice. Its role remains to be further studied.

• 出版日期2018-7