Molecular epidemiology of J-subgroup avian leukosis virus isolated from meat-type chickens in southern China between 2013 and 2014

作者：Lin, Wencheng; Li, Xinjian; Dai, Zhenkai; Zhang, Xinheng; Chang, Shuang; Zhao, Peng; Zhang, Huanmin; Chen, Feng; Xie, Qingmei^*

来源：Archives of Virology, 2016, 161(11): 3039-3046.

DOI：10.1007/s00705-016-3003-8

• POLYMERASE-CHAIN-REACTION
• COMPLETE PROVIRAL GENOME
• SEQUENCE-ANALYSIS
• UNITED-STATES
• LAYER FLOCKS
• HEMANGIOMA
• FIELD
• HPRS-103
• ELEMENT
• STRAIN

Members of avian leukosis virus subgroup J (ALV-J) cause various diseases associated with tumor formation and decreased fertility, resulting in major economic losses in the poultry industry worldwide. To assess the status of ALV-J infection in meat-type chickens in southern China, the molecular epidemiology of ALV-J strains was investigated. A total of 265 clinical samples collected from southern China from 2013 to 2014 were investigated in this study for the presence of ALV-J, which resulted in 12 virus isolates. Phylogenetic analysis showed that 91.7 % (11/12) of the ALV-J isolates have possessed high homology to Chinese layer isolates and belong to one subgroup. One of the ALV isolates (designated GD1411-1) was relatively closely related to the ALV-J broiler isolates, indicating that the GD1411-1 isolate might be a transition strain. Several unique nucleotide substitutions in gp85 and the U3 region were detected in all 12 ALV-J isolates. This study provides some interesting information on the molecular characterization of ALV-J isolates. These findings will be beneficial for understanding of the pathogenic mechanism of ALV-J infection.

• 出版日期2016-11
• 单位华南热带农业大学; 华南农业大学; 山东农业大学