Low pH capillary electrophoresis application to improve capillary electrophoresis-systematic evolution of ligands by exponential enrichment

作者：Li Qian; Zhao Xinying; Liu Hongyang; Qu Feng^*

来源：Journal of Chromatography A, 2014, 1364: 289-294.

DOI：10.1016/j.chroma.2014.08.073

• Protein
• Aptamers selection
• Low pH capillary electrophoresis
• Low pH CE-SELEX

In this work, a novel low pH CE-SELEX (LpH-CE-SELEX) as a CE-SELEX variant is proposed. Transferring (Trf), bovine serum albumin (BSA) and cytochrome c (Cyt c) as model protein are incubated with a FAM labeled ssDNA library, respectively. Incubation mixture is separated in low pH CE (pH 2.6), where positively charged protein, protein-ssDNA complex and negatively charged ssDNA library migrate oppositely without EOF driven. Analysis of protein-ssDNA complex under positive voltage and unbound ssDNA library under negative voltage by CE-UV are applied for interactive evaluation. By increasing injection time, larger amount protein-ssDNA complex can be collected conveniently at the cathode end whereas ssDNA migrates to anode. Finally, stability of protein-ssDNA complex in low pH CE separation is discussed.

• 出版日期2014-10-17
• 单位北京市理化分析测试中心; 北京理工大学