The first true obligately syntrophic propionate-oxidizing bacterium, Pelotomaculum schinkii sp nov., co-cultured with Methanospirillum hungatei, and emended description of the genus Pelotomaculum

作者：de Bok FAM^*; Harmsen HJM; Plugge CM; de Vries MC; Akkermans ADL; de Vos WM; Stams AJM

来源：International Journal of Systematic and Evolutionary Microbiology, 2005, 55(4): 1697-1703.

DOI：10.1099/ijs.0.02880-0

A Gram-positive, spore-forming, syntrophic propionate-oxidizing bacterium, Pelotomaculum schinkii sp. nov. strain HHT, was isolated as a co-culture with Methanospirillum hungatei JF-1(T) from anaerobic, freeze-dried granular sludge obtained from an upflow anaerobic sludge bed reactor treating sugar beet wastewater. The bacterium converted propionate to acetate in co-culture with Methanospirillum hungatei JF-1(T) or Methanobacterium formicicum MFNT, but not in co-culture with Methanobrevibacter arboriphilus AZ. The organism could not be cultured axenically with any of the substrates tested and therefore can be considered as a (the first) true anaerobic syntrophic bacterium. The bacterium contained two distinct 16S rRNA gene sequences, with 96.8% sequence similarity, which were both expressed during syntrophic growth on propionate as revealed by fluorescent in situ hybridization. The most closely related organisms are Cryptanaerobacter phenolicus LR7.2(T), a bacterium that transforms phenol into benzoate, and Pelotomaculum thermopropionicum SIT, a thermophilic, syntrophic propionate-oxidizing bacterium. Other related species belong to the Gram-positive, sulfate-reducing genus Desulfotomaculum. The type strain of Pelotomaculum schinkii is strain HHT (= ATCC BAA-615(T) = DSM 15200(T)).

• 出版日期2005-7