Toll-like receptor 9 (TLR9) gene polymorphisms associated with increased susceptibility of human papillomavirus-16 infection in patients with cervical cancer

作者：Lai Zeng Zhen; Ni Zhang; Pan Xiao Ling^*; Song Liang

来源：Journal of International Medical Research, 2013, 41(4): 1027-1036.

DOI：10.1177/0300060513483398

• Toll-like receptor 9 (TLR9)
• gene polymorphism
• rs352140
• single nucleotide polymorphism (SNP)
• Human papillomavirus (HPV)
• cervical cancer

Objectives: To investigate the association between toll-like receptor 9 (TLR9) single nucleotide polymorphisms (SNPs) and human papillomavirus (HPV) infection among Chinese Han women with cervical cancer.
Methods: TLR9 -1486 and 2848 SNPs were investigated in patients with cervical cancer and controls using polymerase chain reaction (PCR)-restriction fragment length polymorphism. HPV16 E6 and E7 infections were assessed using PCR.
Results: Of 120 patients with cervical cancer and 100 controls, there was a significant association between TLR9 2848 SNP and cervical cancer risk, but there was no such association with TLR9 -1486 SNP. Frequency of the TLR9 2848 GA genotype was significantly higher in patients with cervical cancer than in controls. There was no statistically significant between-group difference in presence of HPV16 infection. Presence of HPV infection with TLR9 2848 (rs352140) GA/AA genotype increased the risk of cervical cancer 13.8-fold compared with the GG genotype.
Conclusions: The TLR9 2848 G/A polymorphism in Chinese Han women was associated with increased risk of cervical cancer in the presence of HPV16 infection. Further studies are necessary to uncover the functional aspect of this TLR9 2848 polymorphism.

• 出版日期2013-8
• 单位四川大学