Antitumor effects of (S)-HDAC42, a phenylbutyrate-derived histone deacetylase inhibitor, in multiple myeloma cells

作者：Bai Li Yuan; Omar Hany A; Chiu Chang Fang; Chi Zeng Pang; Hu Jing Lan; Weng Jing Ru^*

来源：Cancer Chemotherapy and Pharmacology, 2011, 68(2): 489-496.

DOI：10.1007/s00280-010-1501-z

• Histone deacetylase
• (S)-HDAC42
• Myeloma
• Akt
• NF-kappa B
• Cell cycle

Epigenetic agents are among the newly targeted therapeutic strategies being studied with intense interest for patients with multiple myeloma. Here, we demonstrate the antitumor activity of a phenylbutyrate-based histone deacetylase (HDAC) inhibitor, (S)-HDAC42, and identify its possible targets in myeloma cells.
The antiproliferative effect of (S)-HDAC42 was compared with suberoylanilide hydroxamic acid (SAHA) in three myeloma cell lines, IM-9, RPMI-8226, and U266. Flow cytometry and terminal transferase dUTP nick-end labeling (TUNEL) assay were used to demonstrate the induction of apoptosis by (S)-HDAC42. Moreover, the proposed mechanisms of action, such as modulation of Akt, NF-kappa B pathway, and cell cycle-related proteins, were investigated by western blotting.
(S)-HDAC42 exhibited four- to sevenfold higher potency relative to SAHA in suppressing myeloma cell viabilities. The apoptotic effect induced by (S)-HDAC42 was through both intrinsic and extrinsic pathways, as evidenced by increased cleavage of caspase-3, caspase-8, and caspase-9 and release of cytochrome c from mitochondria. In addition to HDAC inhibition, (S)-HDAC42 also disturbed signaling pathways governing cell survival, including downregulating Akt phosphorylation and NF-kappa B signaling. The modulation of cell cycle-related proteins by (S)-HDAC42 suggested its inhibitory effect on cell cycle propagation.
These data suggest the translational value of (S)-HDAC42 in developing new therapeutic strategies for myeloma, which warrants further investigations.

• 出版日期2011-8
• 单位中国医科大学