Notch directly regulates Gata3 expression during T helper 2 cell differentiation

作者：Fang Terry C; Yashiro Ohtani Yumi; Del Bianco Cristina; Knoblock Dawson M; Blacklow Stephen C; Pear Warren S^*

来源：Immunity, 2007, 27(1): 100-110.

DOI：10.1016/j.immuni.2007.04.018

Notch signaling plays multiple roles to direct diverse decisions regarding cell fate during T cell development. During helper T (Th) cell differentiation, Notch is involved in generating optimal Th2 cell responses. Here, we present data investigating how Notch mediates Th2 cell differentiation. Notch showed a CD4+ T cell intrinsic role in promoting IL-4 expression that required GATA-3. In the absence of Notch signals, Gata3 expression was markedly diminished. Introduction of an activated allele of Notchl into CD4+ T cells led to the specific and direct upregulation of a developmentally regulated Gata3 transcript that included the exon 1a sequences. Furthermore, Notch acted in parallel with GATA-3 to synergistically activate IL-4 expression. Together, these data implicate Gata3 as a direct transcriptional Notch target that acts in concert with Notch signaling to generate optimal Th2 cell responses.

• 出版日期2007-7
• 单位河北医科大学