Advances in liquid chromatography coupled to mass spectrometry for metabolic phenotyping

作者：Rainville Paul D; Theodoridis Georgios; Plumb Robert S; Wilson Ian D^*

来源：TrAC-Trends in Analytical Chemistry, 2014, 61: 181-191.

DOI：10.1016/j.trac.2014.06.005

• Global metabolic profiling
• Hydrophilic interaction chromatography
• Liquid chromatography
• Mass spectrometry
• Metabolic phenotyping
• Metabolomics
• Metabonomics
• Reversed-phase liquid chromatography
• Ultra-high-performance liquid chromatography
• Ultra-performance liquid chromatography

We review the separations currently employed in the field of global metabolic profiling and based on the use of liquid chromatography (LC) coupled to mass spectrometry. Currently, most LC separations are performed using reversed-phase (RP) methods, with hydrophilic interaction chromatography a popular choice for polar compounds that are not well served by the RP mode. The use of ultra-(high)-performance LC [U(H)PLC] is seen to be increasingly replacing conventional high-performance LC (HPLC) in metabonomic/metabolomic applications as its benefits are seen as essential for rapid, high-resolution sample analysis. We discuss alternative, and emerging, methods of sample profiling, based on the miniaturization of HPLC-type separations, or the application of supercritical high-performance and ultra-high-performance chromatographic separation.

• 出版日期2014-10