NGF-induced reduction of an outward-rectifying TRPM7-like current in rat CA1 hippocampal neurons

作者：Tian, Shun-Lian; Jiang, Hui; Zeng, Yan; Li, Ling-Li; Shi, Jing^*

来源：Neuroscience Letters, 2007, 419(2): 93-98.

DOI：10.1016/j.neulet.2007.04.020

• nerve growth factor
• TRPM cation channels
• hippocampus
• TrkA
• rat
• patch clamp

Transient receptor potential melastatin 7 (TRPM7) channels are widely expressed in the nervous system; : however, their function and regulation are largely unknown. This study aimed to explore whether the current mediated by TRPM7-like channels in rat CA1 hippocampal neurons could be modulated by nerve growth factor (NGF). Using whole-cell patch clamp techniques, we identified an outward-rectifying TRPM7-like current in hippocampal neurons freshly isolated from postnatal (10-day-old) rats. The outward component of this current was reversibly reduced by NGF in dose- and time-dependent manners, and this effect was substantially blocked by K252a, an inhibitor of TrkA. In addition, NGF-induced reduction of the TRPM7-like current was abolished by U73122, a phopholipase C inhibitor. In light of the abundance of NGF in hippocampus that express both TrkA and TRPM7, these results suggest that the function of TRPM7-like channels in hippocampal neurons may be regulated by NGF.

• 出版日期2007-5-29
• 单位华中科技大学