Detection of African animal trypanosomes: The haematocrit centrifugation technique compared to PCR with samples stored on filter paper or in DNA protecting buffer

作者：Moti Y; Fikru R; Buescher P; Van Den Abbeele J; Duchateau L; Delespaux V^*

来源：Veterinary Parasitology, 2014, 203(3-4): 253-258.

DOI：10.1016/j.vetpar.2014.04.014

• Trypanosoma
• Buffer
• Ethiopia
• Cattle
• Trypanosoma vivax
• Trypanosoma congolense

The present study aimed at comparing the trypanosome specific 18S-PCR-RFLP using samples stored either on Whatman filter papers (PCR-RFLP-fp) or in a commercial cell lysis and DNA protecting buffer (PCR-RFLP-pb) with the haematocrit centrifugation technique (HCT), a method widely used for the diagnosis of African Animal Trypanosomosis. Out of 411 head of cattle, 49 (11.92%) (CI = 8.95-15.45) scored positive for the presence of trypanosomes by HCT whereas 75 (18.25%) (CI = 14.63-22.33) and 124 (30.17%) (CI = 25.77-34.86) scored positive using PCR-RFLP-fp and PCR-RFLP-pb, respectively. Out of the 49 positives by HCT, 14 (28.57%) (CI = 16.58-43.26) and 28 (57.14%) (CI = 42.21-71.18) were concordant by PCR-RFLP-fp and PCR-RFLP-pb, respectively. None of the PCR techniques detected parasites from the Trypanozoon group. Although HCT detected more cases of Trypanosoma vivax (33), species identification using PCR-RFLP-fp and PCR-RFLP-pb were significantly different (p < 0.001) from the HCT technique. The use of DNA protective buffer is thus recommended as the output of the PCR-RFLP-pb is improved and the risk of contamination between samples is reduced.

• 出版日期2014-7