A random amplified polymorphic DNA (RAPD) molecular marker linked to late-bolting gene in pak-choi (Brassica campestris ssp chinensis Makino L.)

作者：Du Zhengxiang; Hou Ruixian; Zhu Yuying^*; Li Xiaofeng; Zhu Hongfang; Wang Zhenzhen

来源：African Journal of Biotechnology, 2011, 10(41): 7962-7968.

• Pak-choi
• late bolting
• random amplified polymorphic DNA (RAPD)
• bulked segregant analysis (BSA)

Bulked segregant analysis (BSA) and random amplified polymorphic DNA (RAPD) methods were used to analyze F(2) individuals of P-27 x P-28 to screen and characterize the molecular marker linked to late-bolting gene in pak-choi (Brassica campestris ssp. chinensis Makino L.). A total of 200 random primers were used for RAPD analysis. One RAPD marker S265(750) was identified to be co-segregating with the late-bolting gene, and the genetic distances between S265(750) and late-bolting gene was 3.14 cM. The results of the study can be seen as a starting point for future researches on the pak-choi bolting gene mapping and molecular marker assisted breeding.

• 出版日期2011-8-3
• 单位上海市农业科学院