Acousto-optic tunable filter-surface plasmon resonance immunosensor for fibronectin

作者：Tian Y; Chen YH; Song DQ; Liu X; Bi SY; Zhou X; Cao YB; Zhang HQ^*

来源：Analytica Chimica Acta, 2005, 551(1-2): 98-104.

DOI：10.1016/j.aca.2005.07.017

• AOTF
• SPR
• immunosensor
• enhanced immunoassay
• fibronectin

An acousto-optic tunable filter-surface plasmon resonance (AOTF-SPR) immunosensor based on wavelength-modulation was applied to detect fibronectin by direct, sandwich and colloidal Au-enhanced immunoassay. The design of the wavelength-modulation AOTF-SPR immunosensor is based on fixing the incident angle of light and measuring the reflected intensity of light in the wavelength range spanning 440-790nm. Fibronectin was determined in the concentration range 2.5-30, 0.5-30, and 0.25-30 mu g/mL for direct, sandwich and colloidal Au-enhanced immunoassay, respectively. The results demonstrate that AOTF-SPR biosensor can be applied to direct and enhanced immunoassay of biomolecule.

• 单位
  吉林大学