Reduction of water evaporation in polymerase chain reaction microfluidic devices based on oscillating-flow

作者：Polini Alessandro; Mele Elisa; Sciancalepore Anna Giovanna; Girardo Salvatore; Biasco Adriana; Camposeo Andrea; Cingolani Roberto; Weitz David A; Pisignano Dario^*

来源：Biomicrofluidics, 2010, 4(3): 036502.

DOI：10.1063/1.3481776

• biological techniques
• biomedical equipment
• bioMEMS
• drops
• elastomers
• evaporation
• fluid oscillations
• lab-on-a-chip
• microchannel flow
• two-phase flow
• water

Producing polymeric or hybrid microfluidic devices operating at high temperatures with reduced or no water evaporation is a challenge for many on-chip applications including polymerase chain reaction (PCR). We study sample evaporation in polymeric and hybrid devices, realized by glass microchannels for avoiding water diffusion toward the elastomer used for chip fabrication. The method dramatically reduces water evaporation in PCR devices that are found to exhibit optimal stability and effective operation under oscillating-flow. This approach maintains the flexibility, ease of fabrication, and low cost of disposable chips, and can be extended to other high-temperature microfluidic biochemical reactors.

• 出版日期2010-9