Dual-wavelength digital holographic imaging with phase background subtraction

作者：Khmaladze Alexander^*; Matz Rebecca L; Jasensky Joshua; Seeley Emily; Holl Mark M Banaszak; Chen Zhan

来源：Optical Engineering, 2012, 51(5): 055801.

DOI：10.1117/1.OE.51.5.055801

• digital holography
• three-dimensional microscopy
• cell imaging
• phase imaging
• phase unwrapping
• background removal
• curvature removal

Three-dimensional digital holographic microscopic phase imaging of objects that are thicker than the wavelength of the imaging light is ambiguous and results in phase wrapping. In recent years, several unwrapping methods that employed two or more wavelengths were introduced. These methods compare the phase information obtained from each of the wavelengths and extend the range of unambiguous height measurements. A straightforward dual-wavelength phase imaging method is presented which allows for a flexible tradeoff between the maximum height of the sample and the amount of noise the method can tolerate. For highly accurate phase measurements, phase unwrapping of objects with heights higher than the beat (synthetic) wavelength (i.e. the product of the original two wavelengths divided by their difference), can be achieved. Consequently, three-dimensional measurements of a wide variety of biological systems and microstructures become technically feasible. Additionally, an effective method of removing phase background curvature based on slowly varying polynomial fitting is proposed. This method allows accurate volume measurements of several small objects with the same image frame.

• 出版日期2012-5