Minimal residual disease of leukemia and the quality of cryopreserved human ovarian tissue in vitro

作者：Asadi Azarbaijani Babak^*; Sheikhi Mona; Nurmio Mirja; Tinkanen Helena; Juvonen Vesa; Dunkel Leo; Hovatta Outi; Oskam Irma C; Jahnukainen Kirsi

来源：Leukemia and Lymphoma, 2016, 57(3): 700-707.

DOI：10.3109/10428194.2015.1065980

• Leukemia
• tissue culture
• ovarian tissue
• minimal residual disease

Auto-transplant of cryopreserved ovarian tissue in leukemia patients carries a risk to reintroduce malignant cells. Maturation of ovarian follicles in vitro is a promising strategy to overcome the leukemic cell contamination. The follicle development and survival in 14 cryopreserved ovarian tissues with leukemia-specific PCR marker was evaluated after 7 or 14 days culture. Minimal residual disease (MRD) quantification was assessed by real-time quantitative PCR in order to identify the MRD positive (n = 6) and negative (n = 8) samples and to monitor levels of MRD before and after culture. The morphology of ovarian follicles were studied by light microscopy. After culture, no statistical significant differences were detected in follicle densities between MRD positive- and negative samples. Ovarian MRD either decreased below undetectable or fluctuated near the baseline level after 7 and 14 days in culture. This study provides quantitative in vitro evidence that leukemia contamination does not affect the follicle survival in cryopreserved ovarian tissue.

• 出版日期2016-3-3