mRNA levels of enzymes and receptors implicated in arachidonic acid metabolism in gliomas

作者：De Armas Rafael; Durand Karine; Guillaudeau Angelique; Weinbreck Nicolas; Robert Sandrine; Moreau Jean Jacques; Caire Francois; Acosta Gisela; Pebet Matias; Chaunavel Alain; Marin Benoit; Labrousse Francois^*; Denizot Yves

来源：Clinical Biochemistry, 2010, 43(10-11): 827-835.

DOI：10.1016/j.clinbiochem.2010.03.017

• Cyclooxygenase
• Lipoxygenase
• Arachidonic acid metabolism
• Astrocytomas
• Oligodendrogliomas
• Gliomas
• Quantitative RT-PCR
• mRNA expression

Background: Gliomas are tumors of the central nervous system derived from glial cells. They show cellular heterogeneity and lack specific diagnostic markers. Although a possible role for the eicosanoid cascade has been suggested in glioma tumorigenesis, the relationship between enzymes and receptors implicated in arachidonic acid metabolism, with histological tumor type has not yet been determined.
Design and methods: Quantitative real-time reverse transcription-polymerase chain reaction was performed to measure and compare transcript levels of enzymes and receptors implicated in both lipoxygenase and cyclooxygenase pathways between oligodendrogliomas, astrocytomas, glioblastomas and mixed oligoastrocytomas.
Results: Arachidonic acid metabolism-related enzymes and receptor transcripts (i) were underexpressed in classical oligodendrogliomas compared to astrocytomas and/or glioblastomas, (ii) differed between astrocytomas and glioblastomas and (iii) had an intermediate expression in mixed oligoastrocytomas.
Conclusions: mRNA levels of enzymes and receptors implicated both in lipoxygenase and cyclooxygenase pathways differed significantly in gliomas according to the histological type.

• 出版日期2010-7