A Gigaseal Obtained with a Self-Assembled Long-Lifetime Lipid Bilayer on a Single Polyelectrolyte Multilayer-Filled Nanopore

作者：Sugihara Kaori; Voeroes Janos; Zambelli Tomaso^*

来源：ACS Nano, 2010, 4(9): 5047-5054.

DOI：10.1021/nn100773q

• planar lipid bilayers
• polyelectrolyte multilayers
• melittin
• single channel monitoring
• impedance spectroscopy

A lipid bilayer with gigaohm resistance was fabricated over a single 800 nm pore in a Si(3)N(4) chip using 50 nm liposomes. The nanopore was prefilled with a polyelectrolyte multilayer (PEM) that triggered the spontaneous fusion of the lipid vesicles. Pore-forming peptide melittin was incorporated in the bilayer, and single channel activities were monitored for a period of 2.5 weeks. The long lifetime of the system enabled the observation of the time-dependent stabilization effect of the melittin open state upon bias application.

• 出版日期2010-9