DETERMINATION OF ARBEKACIN SULFATE INJECTION AND ITS RELATED SUBSTANCES BY HPLC USING EVAPORATIVE LIGHT SCATTERING DETECTION

作者：Wang, Jian; Hu, Xiaojun^*

来源：Journal of Liquid Chromatography & Related Technologies, 2010, 33(4): 441-451.

DOI：10.1080/10826070903574170

• arbekacin sulfate
• arbekacin sulfate injection
• dibekacin
• HPLC-ELSD

A new and simple HPLC-ELSD method for the determination of arbekacin sulfate injection and its related substances was developed. The column was Agilent SB-C18 (250x4.6mm, 5m). The mobile phase was 200mM trifluoroacetic acid - acetonitrile (91:9) flow rate was 0.8mL min-1. The detector used was an Dikma SEDEX 75 ELSD detector. The drift tube temperature was 50 degrees C. The pressure of nebulizing gas was 3.5bar. Good separation of arbekacin from the main related substances could be achieved. The standard curve was rectilinear in the range of 5002500g mL-1 (r=0.9990). The average recovery of arbekacin is 100.8% (R.S.D=1.5%, n=9). The average recovery of dibekacin is 104.7% (R.S.D=2.5%, n=9). The limit of detection of arbekacin was 4.5g mL-1. The limit of detection of dibekacin was 5.0g mL-1. The method is simple and rapid, and the results are accurate and reproducible.

• 出版日期2010
• 单位浙江大学