Detection of Jeotgalicoccus spp. in poultry house air

作者：Martin Elena; Fallschissel Kerstin; Kaempfer Peter; Jaeckel Udo

来源：Systematic and Applied Microbiology, 2010, 33(4): 188-192.

DOI：10.1016/j.syapm.2010.03.008

• Jeotgalicoccus
• Poultry house
• Clone library
• 16S rRNA gene
• Airborne
• Bioaerosol

Investigations of bioaerosols collected from turkey, chicken and duck houses, as well as from a duck slaughterhouse, each in triplicate, revealed that 4-18% of 16S rRNA gene sequences in investigated 16S rRNA gene clone libraries were closely related to Jeotgalicoccus spp. J. halotolerans- and J. psychrophilus-related sequences were obtained in all investigated bioaerosol samples and formed a distinct group with sequences of both species type strains, which were collectively entitled Jeot-cluster-I. For a quantification of Jeot-cluster-I bacteria, a group specific PCR primer combination targeting the 16S rRNA genes was developed. Estimated concentrations by quantitative real-time PCR analyses revealed cell numbers between 10(4) and 10(6) Jeotgalicoccus cells m(-3) air in turkey, duck, and chicken houses, respectively. These results indicated the remarkable proportion (1-39%) of total cell counts and the hitherto unknown wide distribution of Jeotgalicoccus spp. in the poultry rearing industry.

• 出版日期2010-6