Deregulation of microRNAs in blood and skeletal muscles of myotonic dystrophy type 1 patients

作者：Ambrose Kathlin K; Ishak Taufik; Lian Lay Hoong; Goh Khean Jin; Wong Kum Thong; Ahmad Annuar Azlina; Thong Meow Keong^*

来源：Neurology India, 2017, 65(3): 512-517.

DOI：10.4103/neuroindia.NI_237_16

• Biomarker
• microRNA
• myotonic dystrophy type 1
• skeletal muscle
• whole blood

Introduction: MicroRNAs (miRNAs) are short RNA molecules of approximately 22 nucleotides that function as post-transcriptional regulators of gene expression. They are expressed in a tissue-specific manner and show different expression patterns in development and disease; hence, they can potentially act as disease-specific biomarkers. Several miRNAs have been shown to be deregulated in plasma and skeletal muscles of myotonic dystrophy type 1 (DM1) patients. Methods: We evaluated the expression patterns of 11 candidate miRNAs using quantitative real-time PCR in whole blood (n = 10) and muscle biopsy samples (n = 9) of DM1 patients, and compared them to those of normal control samples (whole blood, n = 10; muscle, n = 9). Results: In DM1 whole blood, miRNA-133a, -29b, and -33a were significantly upregulated, whereas miRNA-1, -133a, and -29c were significantly downregulated in the skeletal muscles compared to controls. Conclusions: Our findings align to those reported in other studies and point towards pathways that potentially contribute toward pathogenesis in DM1. However, the currently available data is not sufficient for these miRNAs to be made DM1-specific biomarkers because they seem to be common to many muscle pathologies. Hence, they lack specificity, but reinforce the need for further exploration of DM1 biomarkers.

• 出版日期2017-6