Porcine Circovirus Type 2 Induces Autophagy via the AMPK/ERK/TSC2/mTOR Signaling Pathway in PK-15 Cells

作者：Zhu, Binglin; Zhou, Yingshan; Xu, Fei; Shuai, Jiangbing; Li, Xiaoliang; Fang, Weihuan^*

来源：Journal of Virology, 2012, 86(22): 12003-12012.

DOI：10.1128/JVI.01434-12

• HEPATITIS-C VIRUS
• ACTIVATED PROTEIN-KINASE
• NF-KAPPA-B
• TUBEROUS SCLEROSIS
• TRIGGERS AUTOPHAGY
• REPLICATION
• ERK
• PHOSPHORYLATION
• AMPK
• MACHINERY

Porcine circovirus type 2 (PCV2) uses autophagy machinery to enhance its replication in PK-15 cells. However, the underlying mechanisms are unknown. By the use of specific inhibitors, RNA interference, and coimmunoprecipitation, we show that PCV2 induces autophagy in PK-15 cells through a pathway involving the kinases AMP-activated protein kinase (AMPK) and extracellular signal-regulated kinase 1/2 (ERK1/2), the tumor suppressor protein TSC2, and the mammalian target of rapamycin (mTOR). AMPK and ERK1/2 positively regulate autophagy through negative control of the mTOR pathway by phosphorylating TSC2 in PCV2-infected PK-15 cells. Thus, PCV2 might induce autophagy via the AMPK/ERK/TSC2/mTOR signaling pathway in the host cells, representing a pivotal mechanism for PCV2 pathogenesis.

• 出版日期2012-11
• 单位浙江大学