Hibiscus sabdariffa Polyphenols Alleviate Insulin Resistance and Renal Epithelial to Mesenchymal Transition: A Novel Action Mechanism Mediated by Type 4 Dipeptidyl Peptidase

作者：Peng Chiung Huei; Yang Yi Sun; Chan Kuei Chuan; Wang Chau Jong; Chen Mu Lin; Huang Chien Ning^*

来源：Journal of Agricultural and Food Chemistry, 2014, 62(40): 9736-9743.

DOI：10.1021/jf5024092

• diabetic nephropathy
• epithelial to mesenchymal transition
• insulin resistance
• polyphenol extracts of Hibiscus sabdariffa
• type 4 dipeptidyl peptidase

The epithelial to mesenchymal transition (EMT) is important in renal fibrosis . Ser307 phosphorylation of insulin receptor substrate-1 (IRS-1 (S307)) is a hallmark of insulin resistance. We report that polyphenol extracts of Hibiscus sabdariffa (HPE) ameliorate diabetic nephropathy and EMT. Recently it has been observed that type 4-dipeptidyl peptidase (DPP-4) inhibitor linagliptin is effective for treating type 2 diabetes and albuminuria. We investigated if DPP-4 and insulin resistance are involved in renal EMT and explored the role of HPE. In high glucose-stimulated tubular cells, HPE, like lingaliptin, inhibited DPP-4 activation, thereby regulating vimentin (EMT marker) and IRS-1 (S307). IRS 1 knockdown revealed its essential role in mediating downstream EMT. In type 2 diabetic rats, pIRS-1 (S307) abundantly surronds the tubular region, with increased vimentn in kidney. Both the expression were reduced by HPE. In conclusion, HPE exerts effects similar to those of linagliptin, which improves insulin resistance and EMT, and could be an adjuvant to prevent diabetic nephropathy.

• 出版日期2014-10-8
• 单位河北医科大学