Divergent bufavirus harboured in megabats represents a new lineage of parvoviruses

作者：Sasaki Michihito; Gonzalez Gabriel; Wada Yuji; Setiyono Agus; Handharyani Ekowati; Rahmadani Ibenu; Taha Siswatiana; Adiani Sri; Latief Munira; Kholilullah Zainal Abidin; Subangkit Mawar; Kobayashi Shintaro; Nakamura Ichiro; Kimura Takashi; Orba Yasuko; Ito Kimihito; Sawa Hirofumi^*

来源：Scientific Reports, 2016, 6(1): 24257.

DOI：10.1038/srep24257

Bufavirus is a recently recognized member of the genus Protoparvovirus in the subfamily Parvovirinae. It has been reported that human bufavirus was detected predominantly in patients with diarrhoea in several countries. However, little is known about bufavirus or its close relatives in nonhuman mammals. In this study, we performed nested-PCR screening and identified bufavirus from 12 megabats of Pteropus spp. in Indonesia. Furthermore, we determined nearly the full genome sequence of a novel megabat-borne bufavirus, tentatively named megabat bufavirus 1. Phylogenetic analyses showed that megabat bufavirus 1 clustered with known protoparvoviruses, including human bufavirus but represented a distinct lineage of bufavirus. Our analyses also inferred phylogenetic relationships among animal-borne bufaviruses recently reported by other studies. Recombination analyses suggested that the most common recent ancestor of megabat bufavirus 1 might have arisen from multiple genetic recombination events. These results characterized megabat bufavirus 1 as the first protoparvovirus discovered from megabats and indicates the high genetic divergence of bufavirus.

• 出版日期2016-4-26