MicroRNA microarrays on archive bone marrow core biopsies of leukemias-Method validation

作者：Borze Ioana; Guled Mohamed; Musse Suad; Raunio Anna; Elonen Erkki; Saarinen Pihkala Ulla; Karjalainen Lind**erg Marja Liisa; Lahti Leo; Knuutila Sakari^*

来源：Leukemia Research, 2011, 35(2): 188-195.

DOI：10.1016/j.leukres.2010.08.005

• Bone marrow core biopsy
• Bone marrow aspirate
• miRNA profiling
• ALL
• CML

Due to availability of bone marrow core biopsies (CB) in many pathology laboratories, we evaluated the quality and the biological information of the miRNA profiling using 9 acute lymphoblastic leukemia (ALL) and 9 chronic myeloid leukemia (CML) matched CB and bone marrow aspirates (BA). Technical replicates showed reproducible results across platforms and clustered together in hierarchical clustering analysis; and matched samples showed similar biological content having common differentially expressed miRNAs against the same control samples. We showed, that CBs, which have underwent decalcification in addition to formalin-fixation, are suitable for miRNA profiling.

• 出版日期2011-2