Actin-myosin network is required for proper assembly of influenza virus particles

作者：Kumakura Michiko; Kawaguchi Atsushi^*; Nagata Kyosuke

来源：Virology, 2015, 476: 141-150.

DOI：10.1016/j.virol.2014.12.016

• Blebbistatin
• Influenza virus assembly
• Non-muscle myosin II

Actin filaments are known to play a central role in cellular dynamics. After polymerization of actin, various actin-crosslinking proteins including non-muscle myosin II facilitate the formation of spatially organized actin filament networks. The actin-myosin network is highly expanded beneath plasma membrane. The genome of influenza virus (vRNA) replicates in the cell nucleus. Then, newly synthesized vRNAs are nuclear-exported to the cytoplasm as ribonucleoprotein complexes (vRNPs), followed by transport to the beneath plasma membrane where virus particles assemble. Here, we found that, by inhibiting actin-myosin network formation, the virus titer tends to be reduced and HA viral spike protein is aggregated on the plasma membrane. These results indicate that the actin-myosin network plays an important role in the virus formation.

• 出版日期2015-2