Modulation of immunogenicity of poly(sarcosine) displayed on various nanoparticle surfaces due to different physical properties

作者：Kim Cheol Joo; Hara Eri; Watabe Naoki; Hara Isao; Kimura Shunsaku^*

来源：Journal of Peptide Science, 2017, 23(12): 889-898.

DOI：10.1002/psc.3053

• poly(sarcosine)
• amphiphilic block polypeptide
• nanoparticles
• immunogenicity of nanoparticles
• antibody class-switch

Poly(sarcosine) displayed on polymeric micelle is reported to trigger a T cell-independent type2 reaction with B1a cells in the mice to produce IgM and IgG3 antibodies. In addition to polymeric micelle, three kinds of vesicles displaying poly(sarcosine) on surface were prepared here to evaluate the amounts and avidities of IgM and IgG3, which were produced in mice, to correlate them with physical properties of the molecular assemblies. The largest amount of IgM was produced after twice administrations of a polymeric micelle of 35nm diameter (G1). On the other hand, the production amount of IgG3 became the largest after twice administrations of G3 (vesicle of 229nm diameter) or G4 (vesicle of 85nm diameter). The augmented avidity of IgG3 after the twice administrations compared with that at the single administration was the highest with G3. These differences in immune responses are discussed in terms of surface density of poly(sarcosine) chains, nanoparticle size, hydrophobic component of poly(L-lactic acid) or (Leu- or Val-Aib)(n), and membrane elasticity of the nanoparticles.

• 出版日期2017-12