MOV10 inhibits replication of porcine reproductive and respiratory syndrome virus by retaining viral nucleocapsid protein in the cytoplasm of Marc-145 cells

作者：Zhao, Kuan; Li, Li-Wei; Zhang, Yu-Jiao; Jiang, Yi-Feng; Gao, Fei; Li, Guo-Xin; Yu, Ling-Xue; Zhao, Wen-Ying; Shan, Tong-Ling; Zhou, Yan-Jun; Tong, Guang-Zhi^*

来源：Biochemical and Biophysical Research Communications, 2018, 504(1): 157-163.

DOI：10.1016/j.bbrc.2018.08.148

• Porcine reproductive and respiratory
• syndrome virus
• Moloney leukemia virus 10-like protein
• Nucleocapsid protein
• Viral replication

Porcine reproductive and respiratory syndrome virus (PRRSV) has been a major threat to global industrial pig farming ever since its emergence in the late 1980s. Identification of sustainable and effective control measures against PRRSV transmission is a pressing problem. The nucleocapsid (N) protein of PRRSV is specifically localized in the cytoplasm and nucleus of virus-infected cells which is important for PRRSV replication. In the current study, a new host restricted factor, Moloney leukemia virus 10-like protein (MOV10), was identified as an inhibitor of PRRSV replication. N protein levels and viral replication were significantly reduced in Marc-145 cells stably overexpressing MOV10 compared with those in wild-type Marc-145 cells. Adsorption experiments revealed that MOV10 did not affect the attachment and internalization of PRRSV. Co-immunoprecipitation and immunofluorescence co-localization analyses showed that MOV10 interacted and co-localized with the PRRSV N protein in the cytoplasm. Notably, MOV10 affected the distribution of N protein in the cytoplasm and nucleus, leading to the retention of N protein in the former. Taken together, these findings demonstrate for the first time that MOV10 inhibits PRRSV replication by restricting the nuclear import of N protein. These observations have great implications for the development of anti-PRRSV drugs and provide new insight into the role of N protein in PRRSV biology.

• 出版日期2018-9-26
• 单位中国农业科学院上海兽医研究所; 扬州大学