A replication-time-controlling sequence element in Schizosaccharomyces pombe

作者：Tripathi Vishnu P; Dubey Dharani D^*

来源：Chromosoma, 2017, 126(4): 465-471.

DOI：10.1007/s00412-016-0606-5

• ARS elements
• Replication origins
• Replication timing
• 2D gel analysis
• Fission yeast

Eukaryotic replication origins are highly variable in their activity and replication timing. The nature and role of cis-acting regulatory sequences that control chromosomal replication timing is not well defined. In the fission yeast, Schizosaccharomyces pombe, a 200-bp late-replication-enforcing element (LRE), has been shown to enforce late replication of ARS elements in plasmids. Here, we show that a short (133-bp) fragment of the LRE (shLRE) is required for causing late replication of adjoining origins in its native as well as in an ectopic early-replicating chromosomal location. Active from both sides of an early-replicating origin, the shLRE is a bona fide cis-acting regulatory element that imposes late replication timing in the chromosome.

• 出版日期2017-8