Determination of residues of mebendazole and its metabolites in fish/shrimp by solid phase extraction-high performance liquid chromatography

作者：YU Hui-juan; FENG Bing; JIANG Chao-jun; ZHANG Xiao-ling; HUI Yunhua; SHEN Xiao-sheng

来源：分析试验室, 2010, 29(4).

DOI：10.3969/j.issn.1000-0720.2010.04.009

• Mebendazol
• Metabolites
• Solid-phase extraction
• Residue
• High Performance liquid chromatography

An analytical method was developed to detect residues of mebendazol and its metabolites in fish and shrimp with high performance liquid chromatography-UV detector. The mebendazol and its metabolites were extracted with water and ethyl acetate, defatted with hexane, and purified on a MCX solid phase extraction column. It was studied by sample pre-treating method. The purification efficiency and accurancy of solid phase extraction columns were studied. The recoveries of mebendazol and its metabolites were in the range of 81.4%～95.3% at spiked levels of 5.0～200.0 μg/kg. The relative standard deviations were in the range of 1.9%～8.6%. The linear range of the method was from 10 ng/mL to 2000 ng/mL. The limits of detection for the method are 5.0 μg/kg, 5.0 μg/kg and 10.0 μg/kg for mebendazol, hydoxymebendazole and aminomebendazole, respectively.

• 出版日期2010
• 单位中国水产科学研究院东海水产研究所