科研之友
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摘要
AIM: to determine whether the gastrin stimulated intestinal crypt cell ( IEC-6) proliferation by induction of ornithine decarboxylase, (ODC). METHODS: IEC-6 cells were grown in DMEM containing 50mL . L-1 dialyzed fetal bovine serum for 24 h and then were treated with gastrin. The proliferative capability of the cells was monitored subsequently on d 1, 2, 3, and 4 after treatment with MTT assay at aborbance 570nm. The cellular ODC mRNA expression, ODC activity, and putrescine content were examined by RT-PCR method, radiometric technique and high-performance liquid chromatography (HPLC) analysis respectively after 12h of treatment. RESULTS., On d1 after exposure of IEC-6 cells to pentagastrin, the proliferation increased initially and reached a peak on d3 at 250mug . L-1 concentration. Pentagastrin 500mug . L-1 increased cell proliferation on day 1 and day 2, and then decreased. Compared with control group, pentagastrin 25mug . L-1 increased ODC mRNA level by 1.09-fold ( P < 0.05), ODC activity by 1.71-fold( P < 0.01), and putrescine content 5.30-fold ( P < 0.01) respectively. Similarly, pentagastrin of 500μg . L-1 also increased ODC mRNA level by 1.16-fold ( P < 0.05), ODC activity 1.63-fold( P < 0.05), and putrescine content 4.41-fold ( P < 0.01) respectively. But there was not significant difference between them. CONCLUSION: Gastrin is an agent which promotes IEC-6 cell proliferation involved in regulating ODC activity mechanism.
- 出版日期2002-2
- 单位广州中医药大学
