Analyzing Carbohydrate-Protein Interaction Based on Single Plasmonic Nanoparticle by Conventional Dark Field Microscopy

作者：Jin, Hong-Ying; Li, Da-Wei^*; Zhang, Na; Gu, Zhen; Long, Yi-Tao

来源：ACS Applied Materials & Interfaces, 2015, 7(22): 12249-12253.

DOI：10.1021/acsami.5b02744

• carbohydrate-protein interaction
• single plasmonic nanoparticle
• dark field microscopy

We demonstrated a practical method to analyze carbohydrate protein interaction based on single plasmonic nanoparticles by conventional dark field microscopy (DFM). Protein concanavalin A (ConA) was modified on large sized gold nanoparticles (AuNPs), and dextran Was conjugated on small sized AuNPs. As the interaction between ConA and dextran resulted in two kinds of gold nanoparticles coupled together, which caused coupling of plasmonic oscillations, apparent Color changes (from green to yellow) Of the single AuNPs were observed through DFM. Then, the color information was instantly transformed into a statistic peak wavelength distribution in less than 1 min by a self-developed statistical program (nanoparticleAnalysis). In addition, the interaction between ConA and dextran as proved with biospecific recognition. This approach is high-throughput and real-time, and is a convenient method to analyze carbohydrate protein interaction at the single nanoparticle level efficiently.

• 出版日期2015-6-10
• 单位华东理工大学