Anti-nociceptive effect of total alkaloids isolated from the seeds of Areca catechu L (Arecaceae) in mice

作者：Zhao, Lei^*; Li, Yu; Yang, Shaohui; Zhang, Pei; Wang, Jing

来源：Tropical Journal of Pharmaceutical Research, 2017, 16(2): 363-369.

DOI：10.4314/tjpr.v16i2.15

• Areca catechu
• Total alkaloids
• Anti-nociceptive
• Formalin-induced pain
• Naloxone

Purpose: To investigate the antinociceptive effect of the total alkaloids (TA) isolated from the seeds of Areca catechu L. (SAC) and to elucidate the probable mechanism of action.
Methods: TA extraction conditions including concentration of ethanol, extraction temperature, liquidsolid ratio and designed pH were optimized by an orthogonal experiment {L-9(3)(4)} test. The antinociceptive effect of the extract in mice was evaluated by acetic acid writhing reflex test, hot plate test, capsaicin-induced nociception test, tail-flick test and formalin-induced pain test in mice. Furthermore, pretreatment of the animals with naloxone (2 mg/kg) was performed to investigate whether the antinociceptive effect involved the opioid route or not. The locomotor activity of TA in mice were also assessed.
Results: The optimum extraction conditions of TA were as follows: solid-liquid ratio of 1: 15 (w/v), ethanol concentration of 80 %, pH of 9.0 and extraction temperature of 70 degrees C. Oral administration of TA produced a marked anti-nociceptive activity in mice, and pretreatment with naloxone did not reverse the anti-nociceptive activity of TA in mice. Also, the locomotor activity of mice was not affected by TA. In addition, TA significantly down-regulated the expression levels of COX-2 in the dorsal root of mice spinal cord at 100, 200 and 400 mg/kg doses.
Conclusion: The results demonstrate that TA possesses significant anti-nociceptive effects, and the mechanisms are closely related to suppression of COX-2 expression. Overall, the results provided scientific support for the use of TA in treatment of pain.

• 出版日期2017-2
• 单位河南大学