Comparison of the patterns of antibody recall responses to HIV-1 gp120 and hepatitis B surface antigen in immunized mice

作者：Yu, Hao-Tong; Wang, Jia-Ye; Tian, Dan; Wang, Ming-Xia; Li, Yan; Yuan, Li; Chen, Wen-Jiang; Li, Di; Zhuang, Min^*; Ling, Hong^*

来源：Vaccine, 2016, 34(50): 6276-6284.

DOI：10.1016/j.vaccine.2016.10.063

• HIV-1
• Envelope
• HBsAg
• T follicular helper cells
• PD-1
• Immune memory

To date, we still lack an ideal strategy for designing envelope glycoprotein (Env) vaccines to elicit potent protective antibodies against HIV-1 infection. Since the human hepatitis B virus surface antigen (HBsAg) is representative of effective vaccines that can induce ideal humoral immune responses, knowledge of how it elicits antibody responses and T helper cells would be an useful reference for HIV vaccine development. We compared the characteristics of the HIV-1 Env gp120 trimer and HBsAg in antibody elicitation and induction of T follicular helper (Tfh) and memory B cells in immunized Balb/c mice. Using the strategy of protein prime-protein boost, we found that HIV-1 gp120 induced slower recall antibody responses but redundant non-specific IgG responses at early time after boosting compared to HBsAg. The higher frequency of PD-1(hi)CD4(+) T cells and Tfh cells that appeared at the early time point after gp120 boosting is likely to limit the development of memory B cells, memory T cells, and specific antibody recall responses. These findings regarding the different features of HIV envelope and HBsAg in T helper cell responses may provide a direction to improve HIV envelope immunogenicity.

• 出版日期2016-12-7
• 单位哈尔滨医科大学